The EpiSep XRC™ Slide allows up to 1 ml of sample to be processed per unit. If buffy coat (ficoll fraction) is used, then each unit can process the equivalent of up to 20 ml of whole blood (80-100 billion PBC). Captured tumor cells can then be viewed directly using an inverted epifluorescence or confocal microscope in the same manner as a conventional microscope slide.

Example – EpiSep XRC™, How It Works
Example – Rare Event Detection, Overview

1. Immunomagnetic beads and fluorescent microspheres are added directly to the cell suspension in a tube and incubated under constant rotation.	2. After incubation, the sample is transferred to the well of an EpiSep™ slide. The slide rests on a NeoMag Dock with a magnetic field. Target cells bound by immunomagnetic beads are arrested upon contact with the magnetic field area. Excess liquid and non-targeted cells are absorbed in the pads.
3. Additional Extracellular or intracellular staining of the captured cells is possible on the slides at this point.	4. The EpiSep™ slide is removed from the NeoMag Dock for direct evaluation in an inverted fluorescent microscope. The slides can be handled and stored similar to conventional immunofluorescent slide preparations.

Ref. 060303-124a