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Application to Enrich Rare
Cell Populations - with Fewer Steps
EpiSep™ device for
characterization of breast and lymph node metastasis, stem
cell, and many other applications.
In a few rapid and simple steps
harvest pure populations from tissue, blood, and marrow for
use in cell culture, flow analysis or molecular analysis.
The results of harvesting
SK-Mel-28 melanoma cells performed with EpiSep are shown as
follows:
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| SK-Mel-28 immuno-stained cells captured
by using magnetic particles from EpiSep Melanoma
with DAPI Kit on a micro pillar slide - using a long
pass DAPI filter - 20x |
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SK-Mel-28 immuno-stained cells captured
by using magnetic particles from EpiSep
Melanoma with DAPI Kit on a micro pillar slide - using
a long pass DAPI filter and brightfield illumination -
20x |
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| Transferred cells on magnetic particles
after 1 hour - 20x |
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Transferred cells on magnetic particles
after 1 hour - 40x |
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| Transferred cells on magnetic particles
24 hours - 20x |
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Transferred cells on magnetic particles
after 24 hours - 40x |
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| Transferred cells on magnetic particles
after 120 hours - 10x |
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Transferred cells on magnetic particles
after 120 hours - 20x |
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| Transferred control cells after 24 hours
- 20x |
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Transferred cells treated with magnetic
particle releasing solution after 1 hour - 20x |
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| Transferred cells treated with magnetic
particle releasing solution after 24 hours - 20x |
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Transferred cells treated with magnetic
particle releasing solution after 120 hours - 20x |
As can be
seen from this process, cells are released from the magnetic
capture particles in a manner that allows for further processing
(such as flow cytometry analysis) free of interference.
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