EpiSep CK 8/18 with DAPI is used for
the direct detection of cytokeratin 8/18 positive human epithelial
cells (i.e. circulating tumor cells) from human or animal
whole blood. It is also compatible with ascites, pleural effusions,
buffy coat, Ficoll enrichment and cell culture specimens.
Specificity
The EpiSep Anti-Cytokeratin 8/18 Biotin Conjugate is specific
for human cytokeratins 8 and 18. The immunogens used for this
monoclonal antibody are cytokeratins from the human breast
carcinoma cell line MCF-7. This kit includes DAPI reagent
for identification of nuclei and has been optimized for breast
cancer circulating tumor cell research.
Sensitivity
Direct detection of target cells to levels as low as 1 cell
in up to 1mL f washed whole blood or cell suspensions from
Ficoll, buffy coat, or cell culture.
Recovery
Typical studies using the spiking procedure described here
(link) indicate a recovery of greater than 90%.
