EpiSep Pan Cytokeratin with DAPI is used
for the direct detection of a broad spectrum of positive human
epithelial cells (i.e. circulating tumor cells) from human
or animal whole blood. It is also compatible with ascites,
pleural effusions, buffy coat, Ficoll enrichment and cell
culture specimens.
Specificity The EpiSep Anti-Pan Cytokeratin FITC conjugate recognizes
cytokeratins 4, 5, 6, 8, 10, 13, and 18. This kit includes
DAPI reagent for identification of nuclei and has been optimized
for breast cancer circulating tumor cell research.
Sensitivity Direct detection of target cells to levels as low
as 1 cell in up to 1mL of washed whole blood or cell suspensions
from Ficoll, buffy coat, or cell culture.
Recovery Typical studies using a spiking procedure indicate
a recovery of greater than 90%.
