EpiSep Pan Cytokeratin with DAPI &
MCF-7 Cells is used for the direct detection of a broad spectrum
of positive human epithelial cells (i.e. circulating tumor
cells) from human or animal whole blood. It is also compatible
with ascites, pleural effusions, buffy coat, Ficoll enrichment
and cell culture specimens.
Quality Control Two levels of preserved
MCF-7 epithelial cells in 500uL buffered cell stabilizer are
included in this kit to be tested along with unknown specimens
as a quality control measure.
Specificity The EpiSep Anti-Pan Cytokeratin FITC conjugate recognizes
cytokeratins 4, 5, 6, 8, 10, 13, and 18. This kit includes
DAPI reagent for identification of nuclei and has been optimized
for breast cancer circulating tumor cell research.
Sensitivity Direct detection of target cells to levels as low
as 1 cell in up to 1mL of washed whole blood or cell suspensions
from Ficoll, buffy coat, or cell culture.
Recovery Typical studies using a spiking procedure indicate
a recovery of greater than 90%.
