UltraCytometry™ has ample sensitivity
to provide improved methods for activated T-cells when compared
to flow cytometry. For example, assays that evaluate intracellular
cytokine expression for patients with potential CMV infection
require sufficient sensitivity to be able to identify activated
T-cell populations at levels less than 0.1%. These are sensitivity
levels that are difficult to achieve with flow cytometry,
but are well within the capability of UltraCytometry™.
| Platform |
UltraCytometry™ |
Flow Cytometry* |
| Speed |
Rapid |
Slow |
| Cost |
$ |
$$$$ |
| Usual Number of Cells Sorted |
Millions |
Tens of thousands |
| Sensitivity |
< 0.0001% |
0.1% - 0.01% |
| Technician Expertise Required |
Low |
High |
| FISH Assays |
Yes |
No |
| Cell Recovery |
High |
Moderate |
| Multiple Antigen Analysis |
>10 |
4 – 6 |
| Sterility |
Simple |
Difficult |
| Cell Expansion |
Simple |
Difficult |
| Throughput |
Rapid |
Limited Capacity |
| Tissue Analysis |
Yes |
Limited |
| Cell Engineering |
Simple |
Difficult |
