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| UltraCytometry™ Application Examples |
- UltraCytometry™ provides new tools
for tracking cellular changes such as translation (antigen
expression/immunofluorescence), transcription (mRNA/FISH),
and translocation (DNA/FISH) to optimize high content assays.
- UltraCytometry™ is particularly
useful for determining the effects of siRNA’s as indicated
by observing changes in treated target cell antigen expression.
- UltraCytometry™ can be performed
in a sterile environment which facilitates the rapid implementation
of cell culture, cell signaling, drug resistance and cell
transfection procedures.
- Flow cytometry is routinely used for leukemia and other
relatively target cell rich hematopoietic malignancies,
whereas UltraCytometry™ can be applied to more demanding
assay requirements such as the capture and interrogation
of malignant cells from epithelial tumors, endothelial precursors,
lymphoma, bladder cancer in urine, ductal lavage aspirates
and colonocyte samples.
UltraCytometry™ has ample sensitivity
to provide improved methods for activated T-cells when compared
to flow cytometry. For example, assays that evaluate intracellular
cytokine expression for patients with potential CMV infection
require sufficient sensitivity to be able to identify activated
T-cell populations at levels less than 0.1%. These are sensitivity
levels that are difficult to achieve with flow cytometry,
but are well within the capability of UltraCytometry™.
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